What the run records

Oxygen partial pressure, measured in the sample

A Clark electrode follows PO₂ continuously. Bloodox-2019 pairs that reading with dual-wavelength spectrophotometry, so oxygen partial pressure and hemoglobin oxygenation are stored together.

Binding and release, on one curve

As PO₂ rises, saturation rises and the association curve is recorded. As PO₂ falls, saturation falls and the dissociation curve is recorded. The same sample can be compared across conditions without rebuilding the setup.

Samples

• Whole blood and erythrocyte lysate
• Hemoglobin solution
• Hemoglobin-based oxygen carriers
• Other samples where hemoglobin oxygen binding or release is the question

Experimental control

Gas path, temperature, PO₂, sample handling, and acquisition sit in one instrument, so association and dissociation conditions stay under the same control.

Training videos are sent with the instrument.

Gas flow

Air and nitrogen are set from 0–20 mL/min. Flow rate sets how quickly the sample is oxygenated or deoxygenated.

Temperature

A microcomputer controller holds the sample chamber at 37 ± 0.1 °C, which keeps temperature from shifting the affinity result.

From the touchscreen

• Air or nitrogen selection and gas flow
• Oxygenation and deoxygenation
• Sample fill and flush
• Live PO₂ and temperature

Measurement principle

Deoxyhemoglobin and oxyhemoglobin absorb light differently. Bloodox-2019 follows oxygenation by dual-wavelength spectrophotometry while the Clark electrode measures PO₂. Under air or nitrogen the sample is oxygenated or deoxygenated, and both signals are acquired continuously. The result is the oxygen association or dissociation curve.

Sample chamber

The chamber combines the Clark electrode, temperature sensing, and magnetic stirring. PO₂ and temperature are monitored together, and stirring keeps the sample uniform. Gas, fill, and flush lines are connected to the chamber, so intake, air or nitrogen delivery, and discharge are started from the control panel.

• Automatic sample intake
• Air or nitrogen delivery
• Sample discharge

Data analysis

BL10 records the run. BL10 Viewer processes the curve, calculates the parameters, and compares results.

Association and dissociation curves

The curve is shown as it is acquired. Viewer tools cover the usual cleanup before a parameter is reported:

• Raw Curve
• Translation
• Smooth
• LineFit
• Zero-Completion
• Raw data export

P50

P50 is the PO₂ at 50% hemoglobin oxygen saturation. A higher P50 generally means lower oxygen affinity and a greater tendency to release oxygen. A lower P50 means higher affinity and a greater tendency to bind oxygen.

Hill coefficient

The Hill fit describes cooperativity of hemoglobin–oxygen binding, so samples or conditions can be compared on more than P50 alone.

ROQ

ROQ is the saturation difference between any two PO₂ values you set. It shows how oxygenation changes inside a chosen window, rather than only at 50% saturation.

Several results at once

Curves can be overlaid to compare a left or right shift, P50, affinity, binding, release, and the difference between experimental groups.

Where it is used

The same measurements are used in basic studies of polycythemia, high viscosity, hemolysis, thrombosis, and related questions about how hemoglobin carries oxygen.

Software and system

What is in the instrument

• Dual-wavelength optical detection
• Clark oxygen electrode
• Sample chamber
• Gas-flow control
• Temperature control and temperature sensor
• Magnetic stirring
• Electronics and touchscreen control
• Acquisition and analysis software

Specifications

Selected literature

Methods sections often cite BLOODOX-2018 or Bloodox-2018 (Softron). The current instrument is Bloodox-2019.

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3. Shu, P., et al. Spermine: A Hemoglobin Modifier That Reduces Autoxidation and Regulates Oxygen Delivery. International Journal of Molecular Sciences, 2026. 27(3): 1197.
4. Chu, Z., et al. Simulated Oxygen Supply Efficiency Assessment to Represent Stored Red Blood Cells Quality. Life, 2026. 16(2): 205.
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12. Meng Weicheng, et al. The Impact of Reduced Bisphosphoglycerate Mutase (BPGM) Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity. Journal of Clinical Transfusion and Laboratory Medicine, 2024. 26(3): 308–314.
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17. Li, B., et al. PEG-conjugated bovine haemoglobin enhances efficiency of chemotherapeutic agent doxorubicin with alleviating DOX-induced splenocardiac toxicity in the breast cancer. Artificial Cells, Nanomedicine, and Biotechnology, 2023. 51(1): 120–130.
18. Chu, Z., et al. Resveratrol, a New Allosteric Effector of Hemoglobin, Enhances Oxygen Supply Efficiency and Improves Adaption to Acute Severe Hypoxia. Molecules, 2023. 28(5).
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